Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.
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چکیده
منابع مشابه
Immunocytochemical labelling of haematological samples using monoclonal antibodies.
Monoclonal antibodies have become increasingly important in the diagnosis and classification of neoplastic blood disorders. Immunofluorescent labelling of cell surface antigens has been the conventional method used for this purpose. However, this method of antigen detection presents a number of drawbacks when used in a routine hospital haematology laboratory: namely that blood or bone marrow sa...
متن کاملDouble Labeling of S-Phase Murine Cells with Bromodeoxyuridine and a Second DMA-specific Probe1
A rapid method has been developed which combines immunofluorescence and autoradiography and permits the double labeling of DNA. P388 murine leukemic cells were incubated with bromodeoxyuridine and tritiated thymidine simultaneously. After fixation, the sample was first processed with a monoclonal antibody to bromodeoxyuridine (RPMB I) so that any cell in Sphase was brightly fluorescent (RPMB te...
متن کاملDouble labeling of S-phase murine cells with bromodeoxyuridine and a second DNA-specific probe.
A rapid method has been developed which combines immunofluorescence and autoradiography and permits the double labeling of DNA. P388 murine leukemic cells were incubated with bromodeoxyuridine and tritiated thymidine simultaneously. After fixation, the sample was first processed with a monoclonal antibody to bromodeoxyuridine (RPMB I) so that any cell in S-phase was brightly fluorescent (RPMB t...
متن کاملBromodeoxyuridine in tumors and chromosomes detected with a monoclonal antibody.
Using a monoclonal antibody to bromodeoxyuridine (BUdR) and immunohistochemistry, we measured the incorporation of this thymidine analogue into the DNA of human normal and malignant cells exposed in vivo. BUdR given as a constant intravenous infusion for 12 or 24 h daily for up to 13 d resulted in a steady-state plasma level of 10(-6) M during the infusion. We demonstrated extensive incorporati...
متن کاملCell migration and aggregation in the developing telencephalon: pulse-labeling chick embryos with bromodeoxyuridine.
Previous studies had concluded that the avian telencephalon develops according to an outside-in schedule of neurogenesis, with relatively little migration of young neuroblasts past older cells. These previous studies had, however, been based on the "cumulative labeling" method, which is less accurate than the "pulse-labeling" method typically used in mammals. In the present study, we pulse-labe...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1989
ISSN: 0022-1554,1551-5044
DOI: 10.1177/37.10.2476478